Drugs Identification in Urine, Bile and Gastric Contents using Thin Layer Chromatography in Multiple Screening Systems

A method of simultaneous identification of 25 molecules in human urine, bile and gastric contents using liquid-liquid extraction followed by thin layer chromatography (TLC) using multiple screening systems is described. The analytes were extracted at 25°C under isocratic conditions using chloroform after acidification with 1 to 2 drops of HCl 6 N for 10 mL of […]

Read More

Thin–layer Chromatography (TLC)

Introduction Thin–layer chromatography (TLC) is a widely used technique for the separation and identification of drugs. It is equally applicable to drugs in their pure state, to those extracted from pharmaceutical formulations, to illicitly manufactured materials and to biological samples. TLC as we know it today (see Fig 1) was established in the 1950s with […]

Read More