A method of simultaneous identification of 25 molecules in human urine, bile and gastric contents using liquid-liquid extraction followed by thin layer chromatography (TLC) using multiple screening systems is described. The analytes were extracted at 25°C under isocratic conditions using chloroform after acidification with 1 to 2 drops of HCl 6 N for 10 mL of […]
All posts by Ayoub BENSAKHRIA
Ayoub BENSAKHRIA
Salicylic Acid
Dermatological Agent, Analgesic Synonyms. Acido Ortóxibenzoico; Acidum Salicylicum; Salizylsäure. Proprietary names. It is an ingredient of many proprietary preparations—see Martindale, The complete drug reference, 33rd Edn, London, Pharmaceutical Press, 2002. 2-Hydroxybenzoic acid C7H6O3=138.1 CAS—69–72–7 Colourless, feathery crystals or a white crystalline powder. M.p. 159°. Soluble 1 in about 550 of water, 1 in about 4 […]
Capillary Electrophoresis for Drug Analysis
In the second edition (1986) of this reference work, the index doesn’t even mention electrophoresis. Traditional electrophoretic methodologies, although they used simple and reliable equipment, were always limited by their low resolution of analytes, low throughput, the need to visualise the separated bands and the qualitative nature of the results. They were, in fact, the […]
High Performance Liquid Chromatography (HPLC)
The ability to separate and analyse complex samples is integral to the biological and medical sciences. Classic column chromatography has evolved over the years, with chromatographic innovations introduced at roughly decade intervals. These techniques offered major improvements in speed, resolving power, detection, quantification, convenience and applicability to new sample types. The most notable of these […]
Thin–layer Chromatography (TLC)
Introduction Thin–layer chromatography (TLC) is a widely used technique for the separation and identification of drugs. It is equally applicable to drugs in their pure state, to those extracted from pharmaceutical formulations, to illicitly manufactured materials and to biological samples. TLC as we know it today (see Fig 1) was established in the 1950s with […]