c505218304b50c59c3659f6dda43bae7-links-0–>Immunoassays have a firm place among routine methods for the analysis of drugs in biological fluids and other matrices. The technique may be used by the smallest or largest of laboratories with methods that range from single–use point–of–care tests for the analysis of a single sample to fully automated systems capable of analysing thousands of samples per day. Regardless of format, all immunoassays are based on the interaction of a target molecule (antigen) with a corresponding antibody. When applied to drug testing, the immunoassay technique uses an antibody specific for the drug or drug class being assayed, and a labelled form of the same drug or a labelled form of the antibody to generate a measurable signal. Radioimmunoassay (RIA) is popular because of its high sensitivity, its ability to analyse large numbers of samples rapidly and also because preliminary extraction stages are not required.

Références   [ + ]

1. Clarke’s Analysis of Drugs and Poisons